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Cake day: June 25th, 2023

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  • Explanation: CRISPR-Cas9 is most commonly known to be a gene editor, but since its initial discovery, people have found that with some minor tweaks, CRISPR can be made to do a bunch of other things, things that biologists could have only dreamed of previously.

    If you introduce a mutation that deactivates the Cas9 protein, the entire CRISPR complex still binds to DNA. Then, you can essentially glue whatever you want onto this modified Cas9 to produce a bunch of different effects:

    • deactivated Cas9, no additional modifications: blocks other proteins from touching the DNA, which suppresses a gene without destroying it
    • glue on some transcriptional activators: makes the cell express a gene
    • glue on some fluorescent proteins: makes your DNA glow, lets you see where it is





  • Heavily context dependent, I’d say. In a vacuum, it’s not that unusual. The entire purpose of text is that you don’t have to respond immediately. If it happens constantly, then maybe it would have been worth figuring out why that keeps happening. Maybe he dislikes you, or maybe he’s just busy.

    It seems you already understand that, though. So perhaps the more informative question is why you feel the way that you feel towards your ex. Frankly, it’s probably some level of infatuation (or as I call it, puppy love). It’s not intrinsically bad, but it does tend to drive people to have unrealistic expectations for their partners, which can drive conflict once those expectations become established


  • I struggled with your exact same problem when I moved to Linux. For the record, the reason why there isn’t a Program Files x86 is because the way that Linux stores programs is different from the way that Windows stores programs. Windows sorts its system files by programs, so that the configuration, launcher, and software files are all together. Linux sorts by file type, so that the configuration files of all programs are together, the launchers of all programs are together, etc.

    What I’ve come to learn is, don’t bother trying to find your application in the folder. The best way to install things is through Discover (or whatever GUI software manager you’re using). If the program you want isn’t there, it’s basically a crapshoot whether installing from the internet will work or not








  • To me, it’s a way to quantify how other people feel about my own positions. It’s neither good nor bad to be upvoted or downvoted. People have unpopular takes sometimes and you shouldn’t stake your identity on the amount of upvotes or downvotes you have.

    Instead, upvotes and downvotes are most useful for other people to guage comments. Generally speaking, if a comment is universally downvoted, that likely means the position is unpopular enough that it adds no value to a discussion, and is therefore not worth engaging with.

    I consider it to be a system built upon mutual understanding - that you don’t have to seriously engage with everyone’s viewpoints, and conversely, that nobody has to seriously engage with yours.

    It becomes a problem when upvotes and downvotes are gamified like on Reddit, because Goodhart’s Law demands that it stops serving its purpose when people are only attempting to optimize their upvote/downvote ratio.




  • Probably not a good idea to go for SteamOS. I get the appeal, but I’ve since come to realize that SteamOS isn’t going to work for what we want. It’s designed for handhelds, so it’s never going to be seamless for desktops.

    Valve’s work on SteamOS is open so their work is utilized by a bunch of other distros. So you can actually get the best of both worlds if you expand your options a bit. Pick a popular distro and you’ll be fine


  • For windows-only software, you can keep a copy of windows as a dual boot. Not the most ideal solution, but minimizing windows usage by any little bit decreases the chances of you getting annoyed at Windows.

    Alternatively, if it’s a lightweight software, you could run it in a virtual machine and use something like WinApps to blend it into Linux



  • Contramuffin@lemmy.worldtoScience Memes@mander.xyzPLEASE bro
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    2 months ago

    This is a map of signaling pathways in a cell. Essentially, how does a cell detect a stimulus and how does the cell respond to it. Signaling networks are notoriously difficult to parse, in major part because many of the proteins that make up the network are promiscuous (meaning that they target many types of proteins instead of just 1). Think of network as less of a series of defined steps and more of a chaotic mess. It’s so chaotic that most maps will select a theme (ie, hormone detection, stress signaling, infection, etc.) and only show the interactions that are relevant to that theme. Unfortunately, that also means that each map will show a network that’s completely different from the others, even though the proteins are the same.

    The joke here is that it’s very tempting to make another map. This map is finally going to solve cell biology, I promise, we just have to make another map. Ignore the fact that we already have so many maps, just one more map is all we need